The lines are about 2 1/2 to 3" apart. I used a 9 sharp needle and regular Gutterman sewing thread. I looked for hand quilting thread, but couldn't find a colour that was close enough. I have never hand quilted before, but I used to do a lot of hand embroidery and hand sewing for work. The wool is very lightweight and soft and stitching through it was easy. It was a bit harder to do through the areas with interfacing. Wendy- I didn't use tiger tape, but I did stitch it from the top side. There is a tiny tattersall design in the weave and it gave me lines to follow. It wouldn't be as straight without following that weave.I used the pattern in the weave and tried to get 5 "sections" in each. That isn't five stitches, but 2 overs/3 unders and the reverse.